Justin Timberlake makes very rare appearance with his sons Silas, 11, and Phineas, 6, at charity event in Denver

Justin Timberlake made a rare public appearance with his sons at the 18th annual Be Beautiful Be Yourself fashion show in Denver, Colorado on Saturday, benefitting the Global Down Syndrome Foundation.

The singer, 45, walked the runway with Silas, 11, and Phineas, six, as well as his seven-year-old niece, Zaya, who has Down syndrome and was previously a GLOBAL Ambassador for the organization.

The proud father held hands with Phineas and his niece, while his other son walked on the other side of Zaya while also holding her hand.

Timberlake wore a black jacket and pants, with both his sons matching in black as well. Silas was also seen rocking a pair of black sunglasses on the runway. 

Timberlake - who recently debunked the rumor that his 2006 track What Goes Around... Comes Around was written about ex Britney Spears - shares his sons with wife Jessica Biel, 44, to whom he's been married since 2012.

The hitmaker also posed with Zaya's parents, Rose and Justin Biel, who is the younger brother of his wife.

'Jessica and I adore Zaya, and we couldn’t be prouder of the remarkable difference she is making in the world,' Timberlake said in a statement shared with the organization.

'Every person with Down syndrome deserves the opportunity to reach their full potential, and that’s why GLOBAL's work means so much to our family.' 

Timberlake shared photos from the event to his Instagram Stories, and wrote, 'We walked my beautiful niece Zaya down the @globaldownsyndrome runway last night. Proud uncle!'

He also shared a pic of him and Zaya smiling on the red carpet, and wrote, 'proud uncle!'

Biel was not able to attend this year's event due to work commitments.

She previously walked the runway at the event with both their sons in 2024. 

The event raised $2.7 million to support research, medical care, and advocacy for people with Down syndrome. 

Last month Biel and Timberlake made a show-stopping appearance at the annual Academy Museum Gala.

The longtime couple showed off their bold and eccentric sense of style as they arrived on the red carpet in coordinating all-black looks with different textures.

For the star-studded soirée, Biel wore a halter-style dress with feather details around her neck and a dramatic, fluffy shawl she wore on her arms.

Her dress featured an ultra-high, leg-revealing slit up the side and a long, velvet train that trailed after her as she sauntered across the red carpet.

The 7th Heaven alum paired the daring gown with red bottom stilettos and a messy, slicked-back updo for the event.

On the red carpet, Biel and Timberlake held hands as they posed together.

In October, Biel  opened up about the challenges of balancing her acting career and Timberlake's touring schedule while raising their kids.

While appearing on the SmartLess podcast, she detailed how their family was scattered across continents for weeks at a time. 

'Justin was in the middle of the global tour, which was insane. He was literally in South America... or he was in North America and I was in Budapest with the kids.' 

'They came for seven weeks. We didn't see Justin for eight weeks,' Biel recalled about filming AppleTV's Matchbox: The Movie.

'Then we met back up in the Bahamas for a week. Then I went to Morocco. Then he went to South America and my kids went back to Montana,' she shared.

'It was wild. The only way it works is the incredible nanny team, support team, assistant team, colleague team. I cannot do this. My life does not function without like all of those incredible people,' the working mom emphasized. 

It comes after Biel recently discussed motherhood, over a decade after she welcomed her first child with Timberlake.

As she makes her return to movies, she revealed the reason behind her nine-year hiatus.

'After you have kids, your body does crazy things,' the star explained to People. 'And you’re like, when is this body ever going to feel like mine again?'

'I kind of feel like myself again after 11 years, which sounds crazy,' the mom of two shared.